Michaelis-Menten Enzyme Kinetics

An enzyme catalyzes the conversion of substrate S to product P. The following initial velocities were measured at various substrate concentrations: [S] (μM): 10, 20, 50, 100, 200, 500, 1000 v₀ (μmol/min): 0.50, 0.91, 1.67, 2.22, 2.70, 3.13, 3.33 (a) Estimate Vmax and Km from a Lineweaver-Burk plot. (b) Calculate the turnover number (kcat) if [E]total = 0.1 μM. (c) What is the catalytic efficiency? (d) Is this enzyme considered catalytically perfect?